RT-PCR analysis of SPANX-N expression in normal and tumor tissues and cell lines
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_RT_PCR_analysis_of_SPANX_N_expression_in_normal_and_tumor_tissues_and_cell_lines_/622969
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*SKOV3- ovarian; HeLa-cervix. Each RT-PCR product was cloned in TA vector. From 5 to 40 individual E. coli clones obtained for each sample were analyzed by sequencing. Cloning and sequencing of RT-PCR products determined the gene-specific transcripts in each tissue or cell line. 95 of 103 sequenced clones from different tumor tissues and cancer cell lines corresponded to SPANX-N1. Four of 120 sequenced clones from normal tissues corresponded to SPANX-N1. Expression of SPANX-N2 and SPANX-N3 proteins in lung, testis, placenta and prostate was confirmed by Western blot (Figure 6b).
创建时间：
2015-12-02
