Hemp Seed Oil Inhibits the Adipogenicity of the Differentiation-Induced Human Mesenchymal Stem Cells Through Suppressing the Cannabinoid Type 1 (CB1)

This research focuses on different aspects of hemp seed oil (HSO). Initially, the oil was extracted from shell (S) and no-shell (NS) seeds using different extraction methods and was compared to cold pressed oil in terms of composition. Furthermore, we determine its ability on adipogenic differentiation among hMSCs, and the involvement of the ECS system downstream gene and protein signaling cascade molecules expression levels have been analyzed during the differentiation process. Our results might lead to the involvement of hemp seed and its derivatives in the alteration of the ECS system and its association with lipid metabolism at the early stages of adipocyte differentiation.