Single cell transcriptome analysis of Cyfip2 knockout mouse forebrain

To elucidate the intricate molecular alterations resulting from Cyfip2 knockout, we conducted single cell transcriptomic analysis on the forebrain tissues of E18.5 Cyfip2 knockout mice and their wild-type littermates. The E18.5 stage was selected due to the nonviability of Cyfip2 knockout mice during the perinatal period. Cyfip2 knockout and wild-type mice were obtained by breeding Cyfip2 heterozygous males and females. For each genotype, we combined the forebrains from two mice. Overall design: Single cell transcriptome of embryonic day 18.5 wild-type and Cyfip2 knockout mouse forebrain