Underlying data of Figure 2. Representative cases of the analysis of upstream transcription factors using TFEL against specific promoters.

Underlying data of Figure 2 of "Genome-wide screening of upstream transcription factors using an expression library." <br>(A-C) Representative cases of the analysis of upstream transcription factors using TFEL against specific promoters: (A) vascular endothelial growth factor (gene name: Vegfa), (B) fatty acid synthase (gene name: Fasn), and (C) sterol regulatory element-binding protein-1c (gene name: Srebf1c).<br>The first screening data performed using a 20-clone pool assay are shown (for C: Srebf1c, 10-clone pools were used). Individual clones were identified in the second screening. (D) Representative case identifying the combinatorial interactions on a specific promoter. In this assay, we screened for transcription factor(s) that suppress LXR activity on the SREBP-1c promoter (-249 to -144). To activate LXR and to improve the sensitivity of the assay, we added an LXR ligand T0901317 to the culture media.<br>