SigX ECF sigma factor deletion mutant expression profile in Pseudomonas aeruginosa H103 in LB medium. SigX ECF sigma factor deletion mutant expression profile in Pseudomonas aeruginosa H103 in LB medium

Analysis of a SigX knockout mutant of Pseudomonas aeruginosa H103 strain in LB. Overall design: A deletion mutant of sigX gene (PAOSX) has been studied at the phenotypic level and its expression profile was compared with parental strain Pseudomonas aeruginosa H103 in LB medium. H103 and a sigX mutant were cultured until the milddle of the exponential growth phase. Three independant biological replicate were taken for the RNA extraction and hybridization on affymetrix array.