Transcriptional Effect of 4HTBZ on Caco2 Cells

This dataset comprises four bulk RNA-sequencing files (.fastq.gz) and a metadata file (.csv). Data were obtained from Caco-2/TC7 intestinal epithelial cells pre-stimulated with interferon gamma (IFNG, 2.5 ng/mL) and tumor necrosis factor alpha (TNF, 10 ng/mL), followed by treatment with 4HTBZ (50 µM) or vehicle (0.1% DMSO) for 72 hours. Three biological replicates per condition were pooled prior to RNA extraction. Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany), cDNA libraries were prepared with the QuantSeq 3′ mRNA-Seq Kit (Lexogen, Inc.), and sequencing was performed on an Illumina HiSeq4000 system.
The metadata file provides sample identifiers and corresponding experimental conditions. Raw sequencing reads were assessed with FastQC, processed with Trimmomatic, and aligned to the human reference genome (GRCh38) using HISAT2.
This dataset was generated to characterize transcriptional responses of intestinal epithelial cells to inflammatory stimulation and pharmacological modulation with 4HTBZ.