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miRNA expression profiles in bone marrow mesenchymal stem cell-derived exosomes

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP353716
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SD Rat bone marrow mesenchymal stem cells (BMMSC) were extracted. At the third generation, heme oxygenase-1(HO-1) modified BMMSC (HO-1-MSC) was obtained by transfecting HO-1 with adenovirus. After the cell fusion rate reached 70%, exosome free serum was added. 3 days later, BMMSC and HO-1-MSC cell supernatant were collected, isolated and purified to obtain exosomes. Exosomes were identified by transmission electron microscopy, nanoflow cytometry and westen lot. Exosome sequencing was then performed
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2023-08-08
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