16S rRNA gene sequencing for murine cecal content regarding dietary vitamin A deficiency

To compare our newly-developed cell-based quantitative method, Barcoding Bacteria for Identification and Quantification (BarBIQ) to a conventional 16S rRNA gene amplicon sequencing method, we measured the microbiota of the murine cecum at two locations in C57BL/6J male mice fed with a vitamin A sufficient diet or a vitamin A deficient diet for three weeks by the conventional 16S rRNA gene amplicon sequencing method after removing extracellular DNA.