Proteomic raw data

Extracellular vesicles (EVs) are important for the transport of biologically active materials and function in intercellular communication. Amphibian skin is an exposed mucosa that participates in many important physiological processes. However, little is known about EVs in amphibian skin. Here, using the toad Bombina maxima as the research model, we successfully isolated EVs from skin secretions. These EVs, were characterized using nanoparticle tracking analysis, western blotting and electron microscopy. Proteomic analysis by liquid chromatography with tandem mass spectrometry identified 131 proteins in skin secretion-derived EVs, enriched in proteins related to immune stress, metabolism, oxidoreduction and transport based on their biological processes or molecular functions. The proteins in non-EVs (the proteins in Bombina maxima skin secretions that excepted EV) skin secretions were also analyzed, and 313 proteins were identified in these samples. Comparison of EV and non-EV proteins revealed that 62 proteins were unique to EVs, 244 proteins were unique to non-EVs, and 69 proteins were shared between EVs and non-EV secretions. Flow cytometry assay revealed that the isolated EVs could be taken up by B. maxima skin derived fibroblast cells. These findings provide the first evidence that EVs containing biologically active molecules are secreted from amphibian skin and can be taken up by amphibian skin cells, suggesting that EVs play important physiological roles in amphibian skin.