In vitro selection for chitosan-resistant Staphylococcus aureus - phenotypic and molecular characterisation of resistance development. Staphylococcus aureus

A mutant of SG511 (wild type isolate), resistant to the presence of chitosan (polycationic molecule) was obtained following serial passage experiments resistance to chitosan exposition. Transcriptomic experiments showed alteration in the expression of genes involved in the cell wall regulon resulting in the modification of susceptibility phenotype against cell wall active antibiotics Overall design: Microarray was used to evaluate alteration in the transcriptome between wild tpye strain SG511 and its spontaneous chitosan resistant variant (CRV)