Quantitative Proteomics by Amino Acid Labeling in Foot-and-Mouth Disease Virus (FMDV)-Infected Cells

Foot-and-mouth disease virus (FMDV) is an important disease
agent that can be difficult to effectively eradicate from herds. Because
it is an obligate intracellular parasite, the virus has multiple effects
on the host cell during infection. Here, a high-throughput quantitative
proteomic approach was used to develop an unbiased holistic overview
of the protein changes in IBRS-2 cells infected with FMDV. Stable
isotope labeling with amino acids in cell culture (SILAC) combined
with LC–MS/MS was performed to identify and quantify 1260 cellular
and 2 viral proteins after 6 h of infection of IBRS-2 cells with FMDV.
Of these identified and measured cellular protein pairs, 77 were significantly
up-regulated, and 50 were significantly down-regulated based on significance B ≤ 0.05. The differentially altered proteins included
a number of proteins involved in endolysosomal proteases system, cell
cycle, cellular growth and proliferation, and immune cell trafficking.
Selected data were validated by Western blot. Ingenuity Pathway Analysis
revealed that proteins that changed in response to infection could
be assigned to defined canonical pathways and functional groupings,
such as integrin signaling. The obtained data might not only improve
the understanding of the dynamics of FMDV and host interaction but
may also help elucidate the pathogenic mechanism of FMDV infection.