Metagenome and metatranscriptome sequences from deep-sea hydrothermal vent microbial communities collected on cruises AT42-22, TN405, and NA108 from May 2019 to Jun 2022

This dataset is a collection of sample metadata, identified for all samples, and NCBI accession information for samples and sequence runs produced as part of the \"Microbes need frenemies\" project. This project examines trophic interactions among microbial eukaryotes, viruses, bacteria, and archaea at deep-sea hydrothermal vents using metagenomics and metatranscriptomics and characterizes these ecologically-significant interactions, such as mutualism, predator-prey, or virus-host.

We sequenced samples collected during the 2020 expedition AT42-22 to the Mid-Cayman Rise hydrothermal vent fields, as well as from the 2019 expedition NA108 to the Gorda Ridge and the 2022 expedition TN405 to the Axial seamount. Sequencing targeted archaea, bacteria, and viruses with metagenomics and microbial eukaryotes with metatranscriptomics. We plan to use these data to identify ecologically-significant interactions among protists, viruses, bacteria, and archaea, with a specific emphasis on microbial mortality via viral lysis and eukaryotic grazing. Archived samples were also included in the analysis.