Comparative transcriptomic profiling between wild-type and FvMATE51 mutant (a vacuolar membrane protein) strawberry fruits at 26 DPA

To explore the molecular mechanisms of FvMATE51 during fruit development and ripening, we generated CRISPR/Cas9-mediated gene knockout mutants Fvmate51. Then, we performed comparative transcriptome analysis through RNA sequencing (RNA-seq) using fruits of the wild-type and the mutant lines (Fvmate51-1 and Fvmate51-2) at 26 days post anthesis. Three independent biological replicates were applied for the RNA-seq analysis, using Illumina Nova seq 6000 Overall design: Total RNA was extracted from fruits of 26 DPA WT fruits and FvmMATE1 mutants (Fvmate51-1 and Fvmate51-2) at 26-day postanthesis using HiPure HP Plant RNA Mini Kit (Magen, China).Double-stranded cDNA libraries were prepared from poly(A)+ mRNA, subjected to Illumina high-throughput sequencing after quality control and pooling.