Supplementary Figure 1 Gating strategy for Treg phenotype analysis.
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<strong>Supplementary Figure 1. Gating strategy for Treg phenotype analysis. |</strong> Isolated and purified cells (see Methods) were analysed by flow cytometry with <strong>(A)</strong> gating performed as indicated including the exclusion of dead cells (by Live/Dead staining kit) and analysing only CD4+CD25+ double-positive cells. <strong>(B)</strong> These cells were then stained with indicated marker antibodies. The histogram quantification areas indicated by the black bars were set against control staining performed with non-specific isotype antibodies corresponding to each marker antibody.
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Fruhwirth, Gilbert创建时间：
2022-12-08
