HT1080WT cells - 3D collagen type I matrices (HT1080WT cells embedded in 3D collagen type I matrices - manual annotations for cell instance segmentation and tracking)

嵌入 3D I 型胶原蛋白基质中的低细胞密度的人纤维肉瘤 HT1080WT (ATCC) 细胞 [1]。每 2 分钟录制一次延时视频，持续 16.7 小时，覆盖 1002 像素 × 1004 像素的视场，像素大小为 0.802 μm/像素。对视频进行预处理以纠正帧间漂移伪影，最终尺寸为 983 像素 × 985 像素。 [1] Hasini Jayatilaka、Anjil Giri、Michelle Karl、Ivie Aifuwa、Nicholaus J Trenton、Jude M Phillip、Shyam Khatau 和 Denis Wirtz。 EB1 和细胞质动力蛋白介导突起动力学以实现有效的 3 维细胞迁移。 FASEB J., 32(3):1207–1221, 2018. ISSN 0892-6638。 doi: 10.1096/fj.201700444RR。

Low-cell-density human fibrosarcoma HT1080WT (ATCC) cells embedded in a 3D type I collagen matrix [1]. Time-lapse videos were recorded every 2 minutes for a total duration of 16.7 hours, covering a field of view of 1002 × 1004 pixels with a pixel size of 0.802 μm per pixel. The videos were preprocessed to correct inter-frame drift artifacts, resulting in a final frame dimension of 983 × 985 pixels. [1] Hasini Jayatilaka, Anjil Giri, Michelle Karl, Ivie Aifuwa, Nicholaus J Trenton, Jude M Phillip, Shyam Khatau, and Denis Wirtz. EB1 and Cytoplasmic Dynein Mediate Protrusion Dynamics for Efficient 3D Cell Migration. *FASEB J.*, 32(3): 1207–1221, 2018. ISSN 0892-6638. doi: 10.1096/fj.201700444RR.

搜集汇总

背景与挑战

背景概述

该数据集包含嵌入3D I型胶原蛋白基质中的HT1080WT细胞，提供手动标注的细胞实例分割与跟踪数据。它基于低细胞密度的延时显微镜视频，覆盖16.7小时，像素大小为0.802 μm/像素，适用于细胞迁移研究。