Two cGMP-dependent protein kinases have opposing effects on molt-inhibiting hormone regulation of Y-organ ecdysteroidogenesis

Decapod crustaceans regulate molting through steroid molting hormones, ecdysteroids, synthesized by the molting gland (Y-organ, YO). Molt-inhibiting hormone (MIH), a neuropeptide synthesized and secreted by the eyestalk ganglia, negatively regulates YO ecdysteroidogenesis. MIH signaling is mediated by cyclic nucleotide second messengers. cGMP-dependent protein kinase (PKG) is the presumed effector of MIH signaling by inhibiting mechanistic Target of Rapamycin Complex 1 (mTORC1)-dependent ecdysteroidogenesis. Phylogenetic analysis of PKG contiguous sequences in CrusTome as well as 35 additional species in NCBI RefSeq, identified 206 PKG1 sequences in 108 species and 59 PKG2 sequences in 53 species. These included four PKG1α splice variants in the N-terminal region that were unique to decapods, as well as PKG1β and PKG2 homologs. In vitro assays using YOs from the blackback land crab (Gecarcinus lateralis) and green shore crab (Carcinus maenas) determined the effects of MIH ± PKG inhibito..., , , ## Two cGMP-dependent protein kinases have opposing effects on molt-inhibiting hormone regulation of Y-organ ecdysteroidogenesis

[https://doi.org/10.5061/dryad.1g1jwsv7b](https://doi.org/10.5061/dryad.1g1jwsv7b)

## Description of the data and file structure

This dataset consists of Fasta files of all sequences used for phylogenetic characterization of PKG in crustaceans, and various alignments of isoforms are included; an Excel file with metadata for all sequences used is included and Tree files used to build the PKG phylogenetic tree is included.

Sequencing data for PKG1 isoform validation and PKG2 in G. lateralis and C. maenasAssay data comes from *in vitro* assays of paired Y-organs from *G. lateralis* and *C. maenas* incubated with MIH or MIH ± PKG inhibitors. Hemolymph ecdysteroid (20E) was used to assess the molt stage of the animals, media 20E was measured from the incubation of each YO with the described treatments.

Fasta files include peptide and nucleotide sequences used ...