Pairwise species and site comparisons for gut microbiomes of freshwater mussels collected from six sites in the Sipsey River, AL, USA.

Microbiome comparisons were based on the Bray-Curtis dissimilarity metric. Mussel species are Cyclonaias asperata, Fusconaia cerina, Lampsilis ornata, and Obovaria unicolor and the six sites cover a ~50 km stretch of the river, numbered from upstream to downstream. P-values come from a PERMANOVA comparing bacterial community composition by host mussel species and by site. The test excluded seston samples which were significantly different from each mussel species at each site (p <0.01 for all). The R package pairwiseAdonis was used to perform a post-hoc test to determine which pairwise combinations of species and site were significant. Adjusted p-values of this post-hoc test are displayed below.