Dissecting the Clonal Composition and Determinants of Neutralization Potency Enhancement of Circulating Dimeric and Monomeric IgA to Human Norovirus

We employed serum antibody repertoire proteomic analyses (Ig-Seq) to elucidate the clonal relationships between the antigen-specific circulating mIgA, IgG and dIgA antibody repertoires following oral vaccination with a human norovirus vaccine currently in phase II clinical trials. We then used biochemical and biophysical analyses to define the epitopes and structural determinants that dictate whether and how dIgA can confer enhanced neutralization potency relative to mIgA.