Multimodal spatial omics reveals pro-invasive N-glycosylation in early human placentation

We provide datasets for N-glycomic imaging, transcriptomics and glycoproteomics analyses of first and second trimester decidual tissues. DBDP_glycans.zip contains N-glycomic imaging data and annotation masks of decB (DB) and decP (DP) regions from 3 donor samples. MALDI N-glycan TIF images are in the glycan subfolder. TIF images of each annotation mask region are in the mask subfolder. Annotations are labelled by DBDP_[donor gestational age]_[region#]. Raw intensity of N-glycans in each mask region can be found in the glycan_data.csv table. DBDP_histology.zip contains high resolution H&E and HLA-G IHC slide scans of decidual DB/DP whole tissue sections from 3 donor samples. Each patient sample is labelled DBDP_[donor gestational age]. EVT_glycans.zip contains N-glycomic imaging data and annotation masks of trophoblast regions in decidual tissue microarray (TMA) constructed from 66 donor samples. MALDI N-glycan TIF images are in the glycan_registered subfolder. Annotation mask images are in the mask_registered subfolder. High resolution H&E and HLA-G immunofluorescence scans of the TMA can be found in HnE.ndpi and HLAG_Nuclear_IF.tif respectively. Raw intensity of N-glycans in each mask region can be found in the glycan_data.csv table. EVT_transcript.zip contains Nanostring GeoMx transcriptomics data from selected trophoblast regions of interest in the decidual TMA. Normalized transcript counts are enumerated in normalized_expression.csv, and metadata of regions of interests can be found in metadata.csv. High resolution image of regions of interest overlayed on immunofluorescence staining of morphology markers can in found in overlay_ROI_morphology.png proteomics_glycoproteomics.zip contains processed data for proteomics, deglycoproteomics and EBG-treated glycoproteomics of 5 pairs of patient-matched DB and DP samples. Each data table includes gene names, modified peptide sequence (for deglyco- and glycoproteomics data), log2 intensities in each sample and t-test/limma statistics of each protein or glycopeptide.