Effect of pathway inhibitors on chemokinetic motility of St-T1b cells.

(A) Decidualized St-T1b cells were seeded in Oris migration plates and preincubated with inhibitors for 1 h: PD98059 (50 µM), Y27632 (100 µM), NSC23766 (50 µM), SB202190 (10 µM) or Wortmannin (200 nM). Then PDGF-BB was added to the indicated final concentration, or the equivalent volume of control medium, and cells allowed to migrate into the detection zone for 18 h. Numbers of migrated cells were normalized to the control in the absence of stimulus and inhibitor (left white column). Results represent the means±SEM of n = 3 independent experiments. Data were analyzed by ANOVA and Dunnett test within each group (unstimulated or treated with PDGF-BB) and revealed significant differences after inhibitor treatment compared to the respective controls without inhibitor: *, P<0.05; **, P<0.01; *** P<0.001. (B) Representative images of detection zones for the treatments evaluated in panel (A), showing cells that had migrated into the cell-free zone within 18 h. Cells were stained with Diff-Quik. Images of each detection zone were reassembled from 4 microphotographs taken with a 4× objective and graphically overlaid with the black mask.