Effect of inclusion of pigeon pea leaves in the concentrate mixture on gas production parameters

In vitro gas production technique was employed to evaluate the effect of the inclusion of pigeon pea leaves (PPL) in the CM on gas production kinetics, methane gas emission, ammonia nitrogen production, predicted Metabolizable energy (ME), organic matter digestibility (OMD), microbial protein production (MPP) and short-chain fatty acid (SCFA) production. The experiment was conducted using a completely randomized design with five replications. The treatments were native pasture hay (NPH) and concentrate mixture (CM) at the ratio of 50:50 (T1) or inclusion of 10% PPL in the CM (T2) or inclusion of 20% PPL in the CM (T3) or inclusion of 30% PPL in the CM (T4). The total gas production was greater (P < 0.05) for T1, T2, and T3 than for T4, and total gas production was similar among T1, T2, and T3. The greater (P < 0.05) gas production from immediately soluble fraction was observed for T3 than T4, and T1 and T2 had an intermediate value (P > 0.05). The potential gas production was greater (P < 0.05) for T2 than for T4, while T1 and T3 had an intermediate value. Treatment 1 and T2 had greater (P < 0.05) ME values than Treatment 4, and Treatment 3 had an intermediate value. Organic matter digestibility and microbial N production were increased (P < 0.0001) as the level of inclusion of PPL increased in the CM. It can be concluded that the inclusion of 20% of PPL in the CM was optimum, however, to provide reliable information and to draw a conclusion animal evaluation is required.

体外产气技术被用于评估鸽豆叶（PPL）掺入粗饲料对产气动力学、甲烷气体排放、氨氮生成、可预测的代谢能（ME）、有机物质消化率（OMD）、微生物蛋白生产（MPP）和短链脂肪酸（SCFA）生产的影响。实验采用完全随机设计，重复五次。处理包括原生草地干草（NPH）与浓缩饲料混合物（CM）以50:50的比例（T1）或CM中掺入10%的PPL（T2）或掺入20%的PPL（T3）或掺入30%的PPL（T4）。总产气量在T1、T2和T3中均高于T4（P < 0.05），且T1、T2和T3之间的总产气量相似。在立即溶解组分中，T3的产气量高于T4（P < 0.05），而T1和T2的产气量居中（P > 0.05）。潜在产气量在T2中高于T4（P < 0.05），而T1和T3的产气量居中。处理1和T2的代谢能值高于处理4（P < 0.05），而处理3的产气量居中。随着CM中PPL掺入水平的增加，有机物质消化率和微生物氮生成量均增加（P < 0.0001）。可以得出结论，CM中掺入20%的PPL是最优的，然而，为了提供可靠的信息并得出结论，还需进行动物评估。