Genotyping-By-Sequencing of Aegilops triuncialis derived CSSLs in wheat

In wheat leaf rust and powdery mildew are very destructive diseases. They cause severe yield loses under epidemic conditions. Many chemical methods are available for their management but they cause environmental damage. But use of genetic resistance is environmental friendly as well as economically viable strategy for farmers. For genetic resistance, wild relatives of wheat are very good candidates as they contribute approximately half of the documented R genes for leaf rust and powdery mildew. Among wheat wild relatives Aegilops triuncialis possess resistance against both diseases. Therefore a disomic substitution line was developed in which 5A chromosome of wheat is substituted with 5U chromosome of Ae. triuncialis. To map leaf rust and powdery mildew resistance, chromosome segment substitution lines were generated using pavon ph1b (it lacks Ph1b locus, therefore allows recombination of homeologous chromosomes 5A and 5U) in the background of WL711 cultivar and sent for GBS. Double Digestion Restriction Site Associated DNA Sequencing (ddRAD-Seq) method with two restriction enzymes Sph1 and Mluc1 along with Sequencing Illumina TrueSeq chemistry on Illumina HiSeq X platform was used to generate GBS data of 96 CSSLs.