genome-edited Nicotiana benthamiana plants containing zeaxanthin as the only leaf xanthophyll

We developed transient and stable combinatorial genome editing methods, followed by direct screening for zeaxanthin accumulation, resulting in high efficiency genome editing of the two homeologous Lycopene Epsilon Cyclase and the two Zeaxanthin Epoxidase genes present in the allopolyploid Nicotiana benthamiana genome.