Comparative analysis using different multiplicity of infection ratios from Chlamydia-infected epithelial cells

Here we examined gene expression from an in-vitro dual-RNA-seq experiment using Chlamydia trachomatis-infected HEp-2 epithelial cells. The experimental design consisted of three different multiplicity of infection ratio's (0.1, 1 and 10) across two different timeframes (1 and 24hrs), in addition to applying two different depletion methods (rRNA and rRNA + PolyA depletion). The aim was to examine and outline which MOI and depletion methods are most suited for bacterial-based infections. Examination of chlamydial infection (Chlamydia trachomatis) on human epithelial cells (HEp2) from two timepoints, three MOI ratios and two depletion methods through RNA-Sequencing