Juvenile rainbow trout exposed in situ at site receiving different concentrations of municipal wastewater effluent (MWWE) and an upstream reference site

Juvenile rainbow trout were exposed to MWWE in a caging study, with cages held upstream and downstream of a MWWE outfall for two weeks in the fall of 2007. Whole tissues were sampled and analyzed. Microarray analysis was conducted on liver samples. Four condition experiment, Control (0%),10%, 50% and 100% MWWE. Four independent pooled samples for each treatment, hybridized against a reference pool. The reference pool and test sample were randomly assigned for each array.