Dead-end inhibition patterns and inhibition constants for oxalate and AMP in <i>Tp</i>PK.

Inhibition patterns were taken from the double reciprocal plots of the inhibition experiments (Fig. 3). Simple inhibition patterns were confirmed from linear replots of the slopes or intercepts versus the inhibitor concentrations (not shown). The inhibition constants were calculated from the fits of the complete data set to the corresponding equations for linear competitive inhibition (C) v = V[S]/(Km (1+ [I]/Ki) +[S]), linear noncompetitive inhibition (NC), or linear mixed inhibition (MT) v = V[S]/(Km (1+ [I]/Ki) + [S](1+[I]/αKi)), where α = 1 and α < 1 for NC and MT, respectively; Ki is the inhibition constant. Dead-end inhibition patterns and inhibition constants for oxalate and AMP in TpPK.