<b>Complement inhibition reshapes tumor microenvironment and enhances clinical efficacy in immunotherapy</b>

We conducted single-cell transcriptome (scRNA-seq) and whole-exome sequencing (WES) analyses on 9 esophageal cancer patients, consisting of three cases with complement mutations and six cases without mutations, resulting in a total of 52,727 cells after quality control. The findings indicate a significant increase in the proportion of NK cells and T cells among CD45+ cells in the complement mutation group, whereas the proportion of monocytes and macrophages decreases. Furthermore, gene set enrichment analysis (GSEA) of malignant cells reveals significant enrichment of pathways such as "interferon alpha response," "KRAS signaling DN," and "interferon gamma response" in the complement mutation group.