Response of ancestral and mutant receptors to 11-deoxycorticosterone (DOC).

aAll mutations were introduced into the AncCR background; AncGR1.1 is shown for comparison. Mutations are listed in order of the magnitude of their effect. bThe log effective concentration of hormone for half-maximal activation plus standard error, calculated from nonlinear regression of triplicate reactions in a reporter gene assay. cFold change in EC50 indicates the ratio of EC50 of the listed receptor to the EC50 of AncCR, both for deoxycorticosterone. Positive values indicate reduced sensitivity.