Gene expression analysis of astrocytes differentiated from iPS cells generated from monozygotic twins discordant for schizophrenia

Previous studies have implicated several brain cell types in schizophrenia, but the genetic impact of astrocytes is unknown. Considering their high complexity in humans, astrocytes are likely key determinants of neurodevelopmental diseases. Human induced pluripotent stem cell (hiPSC) -derived astrocytes differentiated from five monozygotic twin pairs discordant for schizophrenia and five healthy subjects were studied for alterations related to high genetic risk and clinical manifestation of SCZ in astrocyte transcriptomics. Ribo-depleted RNA was sequenced with Illumina Next-seq 500 from iPS derived astrocytes