Mettl3 deficiency in epidermal keratinocytes exacerbates psoriasis-like inflammation [MeRIPseq_mice]

We analyzed epidermal samples from keratinocyte-specific knockout Mettl3 (K14CreERT2;Mettl3fl/fl) mice and their controls (Mettl3fl/fl) in imiquimod-induced psoriasis-like skin lesions. Briefly, we established an imiquimod-induced psoriasis model in K14CreERT2;Mettl3fl/fl mice and their controls after tamoxifen injection, and epidermal samples were separated and collected from the skin lesions of the mice after 3 or 6 days of modeling (n=3 per group). The aim of this study was to investigate the mechanism by which Mettl3-mediated epidermal m6A methylation affects skin inflammation. Overall design: At 7 weeks of age, 62.5 mg of IMQ Cream (5%) is applied topically to the shaved backs of mice daily for 3 or 6 days. Dorsal skin samples were excised from adult mice and treated with 3 mg/ml dispase II for 16 hours at 4 °C, and then the epidermis was separated from the dermis under a microscope.