Repairing growth of a genome-reduced strain using adaptive laboratory evolution and targeted gene knock-in

We profiled growth of an evolved version of the genome reduced E. coli strain DGF-298 in various growth conditions. We used RNA-seq to compare this strain's growth to the parent wild-type strain. We also profiled the transcriptional effect of adding in a removed gene predicted by models to have a positive impact on growth (aldA). Overall design: Growth of E. coli was performed with biological duplicates. Total RNA was extracted from mid-log phase culture for RNA-Seq library preparation.