Reversal of human regulatory T cell suppression throgh TLR8 signaling. Reversal of human regulatory T cell suppression throgh TLR8 signaling

Analyses of gene alterations of human Treg cells treated with or without TLR8 ligand using the Illumina whole-genome Human HT-12 BeadChips. The study will provide important information for the molecular signature changes during the reversal of human Treg suppression mediated by activation of TLR8 signaling. Overall design: Human naturally occurring Treg cells were isolated from PBMCs of healthy donors and treated with or without Poly-G3 for 0 and 24 hours. Total RNA was purified from Poly-G3 treated and untreated human Treg cells, and transcriptome analyses of Treg cells were performed using the Illumina whole-genome Human HT-12 BeadChips.