Supplemental material for Non-pasteurized milk enhances early colonization and persistence of Lactobacillus species in dairy calves promoting a stable hindgut microbiome

Figure S1. Fecal microbial composition of the CON and HT groups over time. (a-b) Stacked bar plots of species distribution at phylum and genus levels. (c) Absolute abundance of L. johsonii (U.test, P<0.01).
Figure S2. Effect of pasteurization on the microbiota of milk. (a-b) Composition of colostrum and raw milk microorganisms at the phylum level. (c-d) Determination of viable bacteria in colostrum and milk before and after pasteurization.
Figure S3. Total bacterial isolates and growth phenotypes of L. reuteri (N=40). (a-c) The growth capacity of L. reuteri on glucose. (d-f) The acid production capacity of L. reuteri. (a) The strains were from the feces of CON group calves. (b) The strains were from the feces of HT group calves. (c) The strains were from the unpasteurized milk.
Table S1. Bacterial primers used to estimate the density of bacteria in the calf feces.
A detailed description of each of the 5Qs.