Gene expression data from formalin-fixed paraffin-embedded (FFPE) human uveal melanoma tumors; subset early metastasis vs subset no metastasis

Uveal melanoma (UM) is an aggressive malignancy, in which nearly 50% of the patients die from metastatic disease. Formalin-fixed paraffin-embedded (FFPE) samples represent a valuable source of tumor tissue. Our aim was to investigate differential DNA methylation correlated to gene expression in relation to survival data.We sought to identify aberrant DNA methylation of genes that could be linked to metastatic disease and poor survival. Formalin-fixed parafin-embedded human uveal melanoma tumors; subset early metastasis (n=4) vs subset no metastasis (n=4) were examined by differential gene expression analysis (One-Way ANOVA) to identify 1536 transcripts (1394 up- and 142 down-regulated genes) (p<0.05, FC ≥1.5) . 8 FFPE human uveal melanoma samples; subset early metastasis (n=4) and subset no metastasis (n=4) were used to investigate differential DNA methylation correlated to gene expression in relation to histopathological classification and survival data of human uveal melanoma.We sought to identify aberrant DNA methylation of genes that could be linked to metastatic disease and poor survival.