Investigation of RNA m5C Modifications in Cisplatin-Resistant Ovarian Cancer Cells and the Functional Role of the m5C Reader Protein ALYREF

Cisplatin resistance in A2780 ovarian cancer cells was induced by treatment with gradually increasing concentrations of cisplatin. Transcriptomic differences between cisplatin-resistant and parental A2780 cells were analyzed using RNA sequencing (RNA-Seq), while changes in RNA 5-methylcytosine (m5C) methylation levels were assessed using bisulfite sequencing (BIS-Seq). To investigate the role of the m5C reader protein ALYREF, its expression was knocked down in cisplatin-resistant cells, followed by RNA-Seq to examine resulting gene expression changes. ALYREF-bound RNAs were identified by RNA immunoprecipitation sequencing (RIP-Seq).