Malat1 regulates female Th2 cell cytokine expression through controlling early differentiation and response to IL-2

Transcriptomic profiling of Malat1-deficient and WT CD4+ T cells at Day 0 (naïve CD4+ T cells) and Day 2 post-induction of differentiation under Th2 conditions. Naive CD4+ T cells isolated from spleens and lymphnodes of female mice. For Th2 polarisation, purified naïve CD4+ T cells (500,000 cells per well) were stimulated with 10 µg/ml plate-bound anti-CD3e (clone 145-2C11) and 4 µg/ml soluble anti-CD28 (37.51) in RPMI 1640 in flat-bottom 96-well plates in the presence of 25 ng/ml mouse rIL-4 and 5 µg/ml anti–IFN-? (XMG1.2). Overall design: Examination of expression profiles of splenic and lymph node CD4+ T cells from Day 0 (naive) and Day 2 WT and Malat1-/- cells from female mice using Nanopore RNASeq.