Expression data from U251 RSK1 Knockout and RSK2 Knockout cell lines

The 90 kDa ribosomal S6 kinases (RSKs) are serine threonine kinases comprising four isoforms. The isoforms can have overlapping functions in regulation of migration, invasion, proliferation, survival, and transcription in various cancer types. We delineate RSK isoform-specific transcriptional gene regulation by comparing transcription programs in RSK1 and RSK2 knockout cells using microarray analysis. To determine if RSK1 and RSK2 have specific roles in gene expression, NT, RSK1 knockout and RSK2 knockout U251 cells were for RNA extraction and hybridization on Affymetrix microarrays. Cells were serum starved (0.1% FBS) overnight to minimize RSK activity, then stimulated with epidermal growth factor (EGF) (100 ng/ml) for 5 hours generating 6 samples for analysis: NT-DMSO, NT-EGF, RSK1 Knockout-DMSO, RSK1 Knockout-EGF, RSK2 Knockout-DMSO and RSK2 knockout-EGF.