Tatyana M. Svitkina, Alexander B. Verkhovsky,, Gary G. Borisy (2011) CIL:23050, rattus, fibroblast. CIL. Dataset

Relative distribution of microtubuless and plectin. Electron rnicrograph shows gold-labeled plectin (10 nm) connecting an intermediate filament to microtubules when they
cross each other at different angles. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 7i from J Cell Biol. 1996 Nov;135(4):991-1007.

微管与层粘连蛋白的相对分布。电子显微镜图像展示了用金标记的层粘连蛋白（10纳米）将中间丝与微管连接，当它们以不同角度相交时。细胞骨架的电子显微镜观察方法如Svitkina等（1995年）所述。简而言之，盖玻片上的细胞按光学显微镜的方法进行裂解，用重组的凝胶素N-末端结构域进行处理，用戊二醛、单宁酸和铀酸乙酯固定，临界点干燥，并镀铂和碳。图像对应于《细胞生物学杂志》1996年11月第135卷第4期的第7i图。