Electron microscopy images of thalamus from acutely hydrogen sulfide poisoned mice

To date, the mitochondrial morphological changes that take place after an acute H2S exposure are not well described. In the present study, we analyzed images of transmission electron microscopy of thalami from mice subjected to a single acute (1000 ppm) H2S exposure and euthanized at various time points to assess the mitochondrial structure and morphometric parameters over time. From our observations, changes induced by H2S up to 48 h were mostly limited to increased cristae and matrix compartments' disorganization in mainly accumulated round-shaped mitochondria. At 72 h, H2S induced a spectrum of morphological cellular changes. Impaired H2S-mediated energy-producing capacity of mitochondria relative to their normal-sized counterparts was inferred from our observations of disorganization and paucity of the inner mitochondrial membrane, the infoldings of which—into cristae—are well-documented to increase the surface area for ATP production, the evaluation of a disorganized network, and l..., C57BL/6J male mice 7-8 week old were randomly assigned to each group (n=8 for H2S group and n=4 to room air group). Seven- to eight-week-old mice are considered as young adult mice and used in this study. Mice were exposed to a single dose of H2S at 1000 ppm or room air by inhalation (whole body exposure) for 30 min. This exposure causes 50% mortality (LCt50). Male mice were used because previous work in the lab showed that they are more sensitive to acute H2S exposure than females50. Small sample size of surviving mice (n=3 for each group) were used because this study is a pilot study where electron microscopic investigation was performed for the first time while providing minimal but significant statistical power. Mice in the control group were exposed to room air. High purity of H2S gas (> 99.9 %) or room air (> 98 %) were supplied from gas cylinders (Airgas, IA). The concentration of H2S in the exposure chamber was monitored in real-time using a H2S sensor (RKI Instruments, Un..., Not applicable, # Electron microscopy images of thalamus in brain from acutely poisoned mice with hydrogen sulfide gas

# Dataset contents:

This data set contains 13 raw electron microscopy images that were analyzed to investigate the effects of acute hydrogen sulfide exposure on the brain mitochondria.

# Experimental procedures:

## Exposure paradigm

C57BL/6J male mice 7-8 week old were randomly assigned to each group (n=8 for H2S group and n=4 to room air group). Seven- to eight-week-old mice are considered as young adult mice and used in this study. Mice were exposed to a single dose of H2S at 1000 ppm or room air by inhalation (whole body exposure) for 30 min. This exposure causes 50% mortality (LCt50). Male mice were used because previous work in the lab showed that they are more sensitive to acute H2S exposure than females50. Small sample size of surviving mice (n=3 for each group) were used because this study is a pilot study where electron microscopic investigation was performed for the firs...