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Study Design This study is an experimental post-test-only control group, with anthrax animal model, male white rats (Rattus norvegicus) were used as experimental animal subjects due to their anthrax toxin receptors 2 (ANTXR2), which are adaptable, easy to find, and produce many congenital strains. The derivatives of male white rats (Rattus norvegicus) were already used for various purposes in numerous previous studies, such as behavioral studies or medical testing (13,14). This research took place at Indonesian Agency for Agricultural Research and Development (BALITVET), Bogor, Central experimental animals PAU Universitas Gadjah Mada, Yogyakarta, and the Anatomic Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta. This study uses eight white male rats (Rattus norvegicus) within each group. The research sample was taken from 40 male white rats (Rattus norvegicus) aged three to four months old, weighing 180–200 g. A standard BR I rat feed was used with the adjusted amount to the average body weight. The allocation of experimental animals into each treatment in a homogeneous study was carried out randomly to maintain internal validity. White male rats (Rattus norvegicus) were selected as test animals, due to its’ genetic similarity to humans and adaptability to the laboratory environment (15). Rats that passed the inclusion criteria for this study were fit white male rats with glowing eyes, bright hair, good appetite, active, aged three to four months, and weighed 180–200 g. While rats that didn’t pass the inclusion criteria were white male rats that are sick, with signs of dimmed eyes, low appetite, dull hair, inactive, and underweight. This study used a complete randomized design and carried out an examination at the end of the study and it use animal models of anthrax which are divided into 5 groups, namely P1, anthrax animal model given propolis 200 mg/kg seven days before induction of anthrax spores for up to 14 days; P2, anthrax animal model and given 200 mg/kg of propolis for 14 days; P3, anthrax animal model and was given propolis 200 mg/kg for seven days; P4, anthrax animal model, were given Amoxicillin 9 mg/kg intravenously and were given propolis 200 mg/kg for 14 days; and the group K, anthrax animal model were not given any treatment.