Human Endothelial cell microRNA expression in high and low stiffness

Purpose: mechanical homeostasis is a crucial process for Humen dermal fibroblast cell (HDFs) survival and functionality. Cell can sense environmental change and modify the expression of extracellular matrix, focal adhesion and cytoskeleton protein to maintain the correct mechanical homeostasis. In this study we observed change in microRNA levels linked with mechanical response. HDFs were seeded for 48 our on PDMS 3 kPa and 30 kPa substrate coating with fibronectin to simulate "soft" and "stiff" substrate, then sRNA-seq was performed. Overall design: Human Dermal fibroblastcells (HDFs) were seeded for 48h on substrate at 3 and 30 kPa to approximate “soft” and “rigid” tissue respectively. SRNA-seq was performed to establish the microRNA profile in the two condition