These sequences were checked against the following quality criteria: (i) no Ns, (ii) quality score ≥ 23 according to the PANGEA process (Giongo et al., 2010), (iii) a minimum sequence length of 200 bp
To test the pyrosequencing technology and analysis pipeline under specific conditions, we use a buk defined community with genomic DNAs fromClostridium perfringens ATCC 13124, C. difficile 630, C. ace
Additional file 2. Dataset NRAS-61.txt. A dataset to illustrate the use of the Shiny web interactive application. The dataset includes the pyro-signals (i.e.the raw peak heights) corresponding to four
