Raw data for Figure 4B: Pre-stimulation with IFN-γ, TNF-α and IL-1β does not enhance the immunosuppressive effect of Mesoangioblasts/HIDEMs

HIDEMs/mesoangioblasts were left untreated or were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h before setting up co-cultures with CFSE labelled PBMC and anti CD3/CD28 beads. After 6 days cells were harvested and surface stained for CD3 and 7AAD before analysis of CFSE dilution. CD3+CFSE diluted cell numbers were calculated using counting beads as before. Experiments were carried out in duplicates. n=4.

HIDEMs/间充质干细胞的培养过程中，部分细胞未接受治疗，或经干扰素-γ (IFN-γ)、肿瘤坏死因子-α (TNF-α) 或白介素-1β (IL-1β) (20ng/ml) 刺激24小时后，与用CFSE标记的PBMC及抗CD3/CD28微球共培养。6天后收集细胞，进行CD3和7AAD的表面染色，随后分析CFSE的稀释度。通过计数微球的方法计算CD3+CFSE稀释细胞的数量。实验重复进行，n=4。