cla4d-RNP-CRISPR. Coprinopsis cinerea

NIAID Data Ecosystem2026-05-01 收录

真菌基因编辑

CRISPR-Cas9

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJDB17867 数据链接链接失效反馈

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Strain cla4d-RNP was obtained by RNP-CRISPR method for #292. Strain #292 was transformed using RNP with gRNA for the cla4-ApaI site and pCc1003 (trp1+).

创建时间：

2024-04-06

A Type II-B Cas9 nuclease with no detectable off-targets and reduced chromosomal translocations in vivo.

CRISPR-Cas9

基因治疗安全性

Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as components of CRISPR-Cas9 genome editing and engineering platforms. However, they exhibit promiscuous nuclease activity that

NIAID Data Ecosystem60

The safety of highly stable Cas9 in mice

CRISPR-Cas9

基因编辑动物

Twenty-four male C57BL/6 mice (5 weeks old) were randomly divided into three groups (NC, Cas9, and HSCas9 groups). NC group were hydrodynamically injected with the plasmid pBB4.5-1.3HBV, PX458-Cas9, a

NIAID Data Ecosystem30

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E

CRISPR-Cas9

基因编辑

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E Descriptor: CRISPR-associated endonuclease Cas9/Csn1, DNA (50-MER), RNA (102-MER) Authors: Zheng, R, Ma, L.J. Deposit date: 2025-08-12 Release d

Protein Data Bank Japan2026-05-06 更新20

CRISPR-Cas9 knockout screens using the miRNA-targeting lentiG-miR library

CRISPR-Cas9

miRNA功能研究

The data includes NGS data analyses from CRISPR-Cas9 knockout screens for 47 human cancer cell lines, performed with PoolQ (v2.2.0). This output includes raw read counts of sgRNAs and corresponding qu

Figshare2024-04-25 更新40

Additional file 1: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

基因组编辑

CRISPR-Cas9

Table S1. Sequences of reagents used in the study. The table shows the sequences of the oligonucleotides and lssDNA donors, primers and TaqMan assays employed in this study. LoxP sites (for all condit

Figshare2018-06-26 更新20

cla4d-RNP-CRISPR. Coprinopsis cinerea

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