Viability of progenies.
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At 5–6 h post-mixing, single mating pairs were placed into drops of SPP medium and incubated for 48 h at 30°C. Completion of conjugation development was analyzed by testing for the expression of the drug resistance marker paromomycin in the parental macronuclei, or of 6-methylpurine in the newly developed macronuclei.
创建时间：
2015-12-02
