Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway in budding yeast

The centromeric protein-A (CENP-A) is an evolutionary conserved histone H3 variant that marks the identity of the centromeres. Several mechanisms regulate the centromeric deposition of CENP-A as its mislocalization causes erroneous chromosome segregation, leading to aneuploidy-based diseases, including cancers. The most crucial deposition factor is a CENP-A specific chaperone, HJURP (Scm3 in budding yeast), which specifically binds to CENP-A. However, the discovery of HJURP as a DDR (DNA damage repair) protein and evidence of its binding to Holliday junctions in vitro indicate a CENP-A-deposition-independent role of these chaperones. In this study, using budding yeast, we demonstrate that Scm3 is crucial for the DDR pathway as scm3 cells are sensitive to DNA damage. We further observe that the scm3 mutant genetically interacts with the rad52 DDR mutant and is compromised in activating DDR-mediated arrest. We demonstrate that Scm3 associates with the DNA damage sites and undergoes posttr..., , , # Data from: Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway in budding yeast

[https://doi.org/10.5061/dryad.gb5mkkx1t](https://doi.org/10.5061/dryad.gb5mkkx1t)

## Description of the data and file structure

The genome-wide binding of Scm3 was evaluated from MMS-treated or untreated cells using ChIP-seq assay.

### Files and variables

#### File: Input_MMS_treated-1.fastq.gz

**Description:** Input fasta files from MMS treated sample

#### File: Input_MMS_treated-2.fastq.gz

**Description:** Input fasta files from MMS treated sample

#### File: Input_MMS_untreated-1.fastq.gz

**Description:** Input fasta files from untreated sample

#### File: Input_MMS_untreated-2.fastq.gz

**Description:** Input fasta files from untreated sample

#### File: MMS_treated-1.fastq.gz

**Description:** IP fasta files from MMS treated sample

#### File: MMS_treated-2.fastq.gz

**Description:** IP fasta files from MMS treated sample

#### File: MMS_untreated-1.fastq.gz

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