Table 2 in Diaporthalean fungi associated with canker and dieback of trees from Mount Dongling in Beijing, China

Table 2. Genes used in this study with PCR primers, primer DNA sequence, optimal annealing temperature and corresponding references.
LocusDefinitionPrimersPrimer DNA sequence (5'-3')Optimal annealing temp (°C)References of primers usedITSinternal transcribed spacer of ribosomal RNAITS1TCCGTAGGTGAACCTGCGG51White et al. 1990ITS4TCCTCCGCTTTTGATATGCLSUlarge subunit of ribosomal RNALR0RACCCGCTGAACTTAAGC55Vilgalys and Hester 1990LR7TACTACCACCAAGATCTcalCalmodulinCAL-228FGAGTTCAAGGAGGCCTTCTCCC55Carbone and Kohn 1999CAL-737RCATCTTTCTGGCCATCATGGrpb2RNA polymerase II second largest subunitRPB2-5FGA(T/C)GA(T/C)(A/C)G(A/T)GATCA(T/C)TT(T/C)GG52Liu et al. 1999RPB2-7cRCCCAT(A/G)GCTTG(T/C)TT(A/G)CCCAThis3histone H3CYLH4FAGGTCCACTGGGTGGCAAG58Crous et al. 2004H3-1bGCGGGCGAGCTGGATGTCCTTGlass and Donaldson 1995tef-1αtranslation elongation factor 1-alphaEF1-668FCGGTCACTTGATCTACAAGTGC55Alves et al. 2008EF1-1251RCCTCGAACTCACCAGTACCGtub2beta-tubulinBt2aGGTAACCAAATCGGTGCTGCTTTG55Glass and Donaldson 1995Bt2bACCCTCAGTGTAGTGACCCTTGGC