Dataset used for Estimation of biological variance in coherent Raman microscopy data of two cell lines

We conducted a systematic study to estimate the biological variance in BCARS data of two commonly used cell lines (HEK293 and HepG2) in biomedical research. The BCARS data was acquired from two different experimental setups (Leibniz Institute of Photonics Technology (IPHT) in Jena and Politecnico di Milano (POLIMI) in Milano) to evaluate the reproducibility of results. Also, spontaneous Raman data was independently acquired at POLIMI to validate those results. It has following three folders Raw CARS data POLIMI Raw CARS data IPHT Spontanoues Raman data All the folders include metadata information in a CSV file.