Natural scrapie isolates transmission results to TgOvPrP4 ovine transgenic mice.

(1)Animals were inoculated intracerebrally with 2 mg brain tissue.
(2)Genotype of sheep or goat (amino acids 136, 141, 154, and 171).
(Goat)Goat isolate.
(α)Isolates detected in the flock α.
(β)Isolates detected in the flock β.
Natural inoculums were divided into 4 different groups, all originating from active surveillance of TSEs in small ruminants. Group 1a: atypical scrapie cases with prnp alleles associated with increased susceptibility to atypical scrapie (AF141RQ or AHQ). Group 1b: atypical scrapie cases without prnp alleles associated with increased susceptibility to atypical scrapie. Group 2: classical usual scrapie cases. Group 3: CH1641-like scrapie cases. Detection of PrPres/PrPsc was performed by Western blot or IHC analyses.