Transcriptome analysis of SARS-CoV-2 infected H522 human lung adenocarcinoma cells

Purpose: The goal of this study is to define the transcriptional response of H522 cells to SARS-CoV-2 infection. Methods:H522 cells were infected with SARS-CoV-2 at varying multiplicities of infection and samples processed for RNA-seq at 4, 24, 48, 72 and 96 hours post-infection. Results: We provide the transcriptomic and translatomic landscape of SARS-CoV-2-infected H522 cells from multiple RNA-seq libraries. We found that the robust transcriptional upregulation of numerous type I interferon and cell cycle associated genes in SARS-CoV-2-infected cells. Conclusions: Our study represents the detailed analysis of transcriptional responses of H522 cells to SARS-CoV-2 and demonstrates upregulation of key pathways that may paly a role in disease pathogenesis. Overall design: H522 cells were infected with SARS-CoV-2 at varying multiplicities of infection and samples processed for RNA-seq at 4, 24, 48, 72 and 96 hours post-infection.